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"Genetic Manipulation of DNA and Protein: Examples from Current Research" ed. by David Figurski

"Genetic Manipulation of DNA and Protein: Examples from Current Research" ed. by David Figurski
English | ITAe | 2013 | ISBN: 9789535109945 | 461 pages | PDF | 15 MB
This diverse collection of research articles is united by the enormous power of modern molecular genetics. Every author accomplished two objectives: (1) making the field and the research described accessible to a large audience and (2) explaining fully the genetic tools and approaches that were used in the research.

Read this book, you will learn about an area of research with which you may not be familiar. Perhaps most importantly, you will understand the genetic approaches; and you will appreciate their importance to the research.

Contents
Preface
Section 1 Molecular Genetics in Basic Research
1 Site-Directed Mutagenesis and Yeast Reverse 2-Hybrid-Guided Selections to Investigate the Mechanism of Replication Termination
2 Biochemical Analysis of Halophilic Dehydrogenases Altered by Site-Directed Mutagenesis
3 Targeted Mutagenesis in the Study of the Tight Adherence (tad) Locus of Aggregatibacter actinomycetemcomitans
4 Directed Mutagenesis of Nicotinic Receptors to Investigate Receptor Function
5 Site-Directed Mutagenesis as a Tool to Characterize Specificity in Thiol-Based Redox Interactions Between Proteins and Substrates
6 Protein Engineering in Structure-Function Studies of Vipers Venom Secreted Phospholipases A2
7 Site-Directed Mutagenesis in the Research of Protein Kinases - The Case of Protein Kinase CK2
8 Directed Mutagenesis in Structure Activity Studies of Neurotransmitter Transporters
9 Site-Directed Mutagenesis as a Tool for Unveiling Mechanisms of Bacterial Tellurite Resistance
Section 2 Molecular Genetics in Disease-Related Research
10 A Mutagenesis Approach for the Study of the Structure-Function Relationship of Human Immunodeficiency Virus Type 1 (HIV-1) Vpr
11 New Insights into the Epithelial Sodium Channel Using Directed Mutagenesis
12 Use of Site-Directed Mutagenesis in the Diagnosis, Prognosis and Treatment of Galactosemia
13 Inherited Connective Tissue Disorders of Collagens: Lessons from Targeted Mutagenesis
Section 3 Molecular Genetics in Applied Research
14 Biological Activity of Insecticidal Toxins: Structural Basis, Site-Directed Mutagenesis and Perspectives
15 Site-Directed Mutagenesis as Applied to Biocatalysts
Section 4 New Tools or Approaches for Molecular Genetics
16 Using Cys-Scanning Analysis Data in the Study of Membrane Transport Proteins
17 Site-Directed Mutagenesis Using Oligonucleotide-Based Recombineering
18 Studying Cell Signal Transduction with Biomimetic Point Mutations
19 Using Genetic Reporters to Assess Stability and Mutation of the Yeast Mitochondrial Genome
20 Site-Directed and Random Insertional Mutagenesis in Medically Important Fungi
21 Recombineering and Conjugation as Tools for Targeted Genomic Cloning
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Tags: Genetic, Manipulation, Protein, Examples, Current, Research, Figurski

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